CHRM2/CHO
| I. Background | |
| 毒蕈堿乙酰受體（MAChRs，簡稱 M 受體）參與中樞神經(jīng)系統(tǒng)的運(yùn)動(dòng)控制、體溫 調(diào)節(jié)、心血管調(diào)節(jié)和記憶，介導(dǎo)周圍神經(jīng)系統(tǒng)的平滑肌收縮、腺體分泌和心率、調(diào)節(jié)心肌 收縮力。CHRM2 屬于 GPCR，是 MAChRs 的 5 種亞型之一，可以與 Gi/o 偶聯(lián)。CHRM2 是心臟和腦干中最主要的毒蕈堿受體亞型。CHRM2 通過 Gi/o 信號(hào)精密抑制心臟與神經(jīng)元 過度活動(dòng)，維持內(nèi)環(huán)境穩(wěn)態(tài)。其功能失衡直接導(dǎo)致心動(dòng)過緩、癲癇易感性升高及抗抑郁治 療抵抗，是神經(jīng)心血管藥物設(shè)計(jì)的核心靶標(biāo)。 | |
| II. Description | |
HRM2 CHO 藥靶模型很好的模擬了體內(nèi) CHRM2 的信號(hào)轉(zhuǎn)導(dǎo)過程，原理見下圖所示。 Figure 1. CHRM2 CHO 細(xì)胞模型原理圖 | |
| III. Introduction | |
| Host Cell: | CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5μg/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assays for CHRM2 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
Figure 2. Recombinant CHRM2/CHO constitutively expressing CHRM2. | |
Figure 3. HTRF cAMP Assay With CHRM2 CHO Cells (C3). | |
化工儀器網(wǎng)