| I. Background | |
趨化因子(C-C motif)受體 8 (CCR8)是一種主要表達于調節性 T 細胞(Treg)上的趨化因子 受體，屬于 G 蛋白偶聯受體。CCR8 的配體有 CCL1、CCL8、CCL16 和 CCL18。 | |
| II. Description | |
cAMP 在 G 蛋白偶聯受體(GPCR)信號轉導中是一種關鍵的第二信使。當配體結合到 GPCR，構象發生變化，激活受體接著激活 G 蛋白，Gi 的激活導致腺苷酸環化酶調控 cAMP 的下調。CCR8 藥靶模型很好的模擬了體內 CCR8 的信號轉導過程，原理見下圖所示。
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| III. Introduction | |
| Host Cell: | CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5μg/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for CCR8 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
Figure 2. Recombinant CCR8/CHO constitutively expressing CCR8. | |
Figure 3. HTRF cAMP Assay with CCR8 CHO(C36). | |
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