I. INTRODUCTION
Osteoprotegerin （OPG）ELISA Kit is a protein of 401 amino acids synthesized as a monomer of approximay 55 kDa. The mouse and human OPG proteins are approximay 85 percent and 94 percent identical with the rat protein, respectively. OPG belongs to the superfamily of TNF receptor-like proteins,having a strong similarity with the type-2 TNF receptor and CD40 and has been described as TNF receptor-like-1. OPGL appears to be an osteoclast differentiation and activation factor.

Human Osteoprotegerin ELISA （Enzyme-Linked Immunosorbent Assay） kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Osteoprotegerin in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human Osteoprotegerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteoprotegerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and
biotinylated anti-human Osteoprotegerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is
pipetted to the wells. The wells are again washed, a TMB substrate solution
is added to the wells and color develops in proportion to the amount of
Osteoprotegerin bound. The Stop Solution changes the color from blue to
yellow, Human Osteoprotegerin ELISA kit and the intensity of the color is measured at 450 nm.

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