FuGENE  HD轉染試劑

*30%的轉染用戶使用的FuGENE®，其優勢在于：

· Off-target效應低 – 轉染試劑將外源核酸片段導入細胞的同時，也將影響內源基因表達水平，從而對細胞的代謝和狀態造成不利影響，或影響實驗數據的客觀性，這稱為off-target效應。FuGENE®只是LipofectAMINE 2000的16%，試驗表明，轉染MCF-7 cells （ATCC® HTB-22™）時，采用micro-array檢查發現，使用LipofectAMINE 2000轉染導致細胞內源1741個基因表達發生變化，而使用FuGENE® HD只有282個內源基因表達發生變化。[發表于Biochemica 4, 22-25 （2006），請登www.roche-applied-science.com/publications/biochemica.htm閱讀電子版]

· 細胞毒性低 – 這是下游實驗順利進行的保證

FuGENE® HD轉染試劑

繼堪稱轉染試劑精粹的FuGENE® 6之后，羅氏推出全新的FuGENE® HD，全面提升轉染實驗結果，為您實驗助一臂之力。

· 轉染目前已有的轉染試劑難轉染的細胞株，例如：MCF-7，RAW 267.7，PC-3，Hela，MA-10，HepG2，SH-SY5Y，A7r5，STO，SCC-61，STSAR-90，SQ20B，T98G，STSAR-90，A375，A549等；

· 同時適用于昆蟲細胞，多種癌癥細胞株，原代細胞和干細胞轉染；

· 更低的細胞毒性，有助獲得更多具有活性的功能細胞，避免和減少使用其他轉染試劑導致的對細胞應答和細胞基因表達的影響；

· 顯著提升目的基因在常用的細胞株中的蛋白表達水平，通過調整轉染試劑的用量，就可輕松優化蛋白表達；

· 更安全可靠的組份，不含任何動物來源成份，經0.1um過濾滅菌，100%血清中依然有活性；

· 穩定的試劑，室溫狀態下依然穩定；簡便的操作步驟，快速實驗方案同時適用多種細胞，滿足大通量的實驗操作需要。

ROCHE相關產品資料

FuGENE^® HD Transfection Reagent

FuGENE^® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Application

FuGENE^® HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. It is designed to transfect a wide range of eukaryotic cells including insect cells and many cell lines not transfected well by other reagents （e.g .,  MCF-7, RAW 264.7, PC-3, HeLa, MA-10, HepG2, SH-SY5Y, A7r5, STO, SCC-61, STSAR-90, SQ20B, T98）.

Note: For the most up-to-date list of cell types that have been successfully transfected with FuGENE^® HD Transfection Reagent, visit our Transfection Special Interest Site at www.powerful-transfection.com and view our Transfection Reference Database. This site also contains much more information about the use of this reagent.

For life science research only.

Product Description

Composition: unique and proprietary blend of many components,  in 80% ethanol.

Storage:  stable at +2°C to +8°C.

Stability: 2 years, starting from date of manufacture.

Contents

Solution in 80% ethanol, filter-sterilized （0.1 µm filter）, packaged in transparent glass vials.

Quality

Lot-specific analysis: 
Each lot of FuGENE^®HD Transfection Reagent is carefully checked, using ISO DIN 9001-certified procedures, to assure product is performing according to specifications and is consistent from lot to lot.

Functional analysis:
Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.3 µl FuGENE^® HD and 0.1 µg DNA or 0.25 µl FuGENE^® HD and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be ≥ 75% compared to a reference lot in at least one of the tested amounts, listed above.

Cytotoxicity analysis:
Cell growth is assessed using the Cell Proliferation Reagent WST-1assay  which is an indirect measure of cytotoxicity. The remainder of cells from functional analysis is tested for viability.