性做久久久久久坡多野结衣-性做久久久久久久久浪潮-性欲影院-性影院-国产精品线路一线路二-国产精品兄妹在线观看麻豆

產(chǎn)品展廳收藏該商鋪

您好 登錄 注冊

當(dāng)前位置:
美國布魯克海文儀器公司>技術(shù)文章>Omni測量應(yīng)用案例-2016-21

技術(shù)文章

Omni測量應(yīng)用案例-2016-21

閱讀:307          發(fā)布時間:2016-6-20

文獻(xiàn)名: Chlorin e6 keratin nanoparticles for photodynamic anticancer therapy

 

作者 A. Aluigi,a   G. Sotgiu,a   C. Ferroni,a   S. Duchi,b   E. Lucarelli,b   C. Martini,a   T. Posati,a   A. Guerrini,a   M. Ballestri,a   F. Corticellic and    G. Varchia  

a Institute of Organic Synthesis and Photoreactivity – Italian National Research Council, Via Gobetti 101, 40129 Bologna, Italy

b Osteoarticular Regeneration Laboratory, Rizzoli Orthopaedic Institute, Via di Barbia/10, 40136 Bologna, Italy

c Institute for Microelectronics and Microsystems, National Research Council, Via Gobetti 101, 40129 Bologna, Italy

 

 

摘要:This work describes the preparation of keratin and chlorin e6-conjugated keratin nanoparticles, KNPs and KNPs@Ce6 respectively, by comparing self-assembling and desolvation methodologies. Nanoparticles were characterized in terms of yield, size, morphology, Ce6 loading ratio and ability to produce reactive oxygen species (ROS) upon irradiation with white light. Overall, both methods provided nanoparticles of comparable dimensions, morphology and Ce6 loading ratio. KNPs@Ce6 obtained by a self-assembling procedure were able to produce ROS in a concentration and irradiation-time dependent manner, while displaying compelling evidence of their photostability. In vitro internalization and photo-toxicity studies were performed on osteosarcoma (U2OS) and glioblastoma (U87) cells lines to assess the ability of KNPs@Ce6 nanoparticles to act as delivery systems for photodynamic therapy of cancer. Importantly, at all the Ce6 considered concentrations, e.g. 0.5, 2.5 and 5.0 μg mL−1, no dark toxicity was detected while the amount of Ce6 inside the cells, significantly increased when loaded onto KNPs. The irradiation of tumor cells loaded with KNPs@Ce6 resulted in a greater cell death percentage (approximay 90%) as compared to free Ce6 in both cell types and at all the considered concentrations, thus showing KNPs as effective and promising delivery vehicles for photodynamic therapy applications.

 

收藏該商鋪

登錄 后再收藏

提示

您的留言已提交成功!我們將在第一時間回復(fù)您~

對比框

產(chǎn)品對比 產(chǎn)品對比 聯(lián)系電話 二維碼 意見反饋 在線交流

掃一掃訪問手機(jī)商鋪
010-62081908
在線留言
主站蜘蛛池模板: 清流县| 收藏| 绍兴市| 江北区| 资源县| 望谟县| 普兰店市| 桑日县| 邮箱| 叶城县| 边坝县| 稻城县| 裕民县| 广州市| 抚州市| 鹰潭市| 南康市| 静宁县| 东阳市| 奈曼旗| 绥化市| 昌吉市| 黑山县| 南开区| 平陆县| 营口市| 龙门县| 汕尾市| 科技| 夏邑县| 林甸县| 元江| 永定县| 汉中市| 英吉沙县| 禄劝| 平乐县| 白银市| 闸北区| 百色市| 西藏|