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ZetaPALS Zeta電位測量應用案例-4
閱讀：237 發布時間：2014-9-12
文獻名： Bacterial quorum sensing and cell surface electrokinetic properties
作者： K. E. Eboigbodin, J. R. A. Newton, A. F. Routh, C. A. Biggs
1. Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield, S1 3JD, UK
2. Department of Chemical Engineering and BP Institute, University of Cambridge, Cambridge, CB3 0EZ, UK
摘要：The hypothesis tested in this paper is that quorum sensing influences the microbial surface electrokinetic properties. Escherichia coli MG1655 and MG1655 LuxS- mutant (lacking quorum-sensing gene for Autoinducer synthase AI-2) were used for this study. AI-2 production (or lack of) in both strains was analyzed using the Vibrio harveyi bioassay. The levels of extracellular AI-2 with and without glucose in the growth medium were consistent with previously published work. The surface electrokinetic properties were determined for each strain of E. coli MG1655 by measuring the electrophoretic mobility using a phase amplitude light-scattering (PALS) Zeta potential analyser. The findings show that the surface charge of the cells is dependent upon the stage in the growth phase as well as the ability to participate in quorum sensing. In addition, significant differences in the electrophoretic mobility were observed between both strains of E. coli. These findings suggest that quorum sensing plays a significant role in the surface chemistry of bacteria during their growth.
關鍵詞：Cell surface charge；Electrokinetic potential；Quorum sensing；Escherichia coli；Aggregation