The JMJD2E Homogeneous Assay Kit is designed to measure activity of JMJD2E for screening and profiling applications, using AlphaLISA^® technology. The key to the JMJD2E Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2E enzyme is incubated with the biotinylated substrate for two hours. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alphacounts.