Recombinant clonal stable Jurkat T cell line expressing firefly luciferase gene under the control of 4 copies of NF-κB response elements with constitutive expression of human HVEM (Herpes Virus Entry Mediator, Tumor Necrosis Factor Receptor Superfamily Member 14, TNFRSF14, CD270, GenBank Accession No. NM_003820). Following activation by human HVEM ligａnd LIGHT, NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase reporter gene.Materials Required but Not Supplied: Assay Medium: Thaw Medium 2 (BPS Cat. #60184)LIGHT, His-Tag (Human) (BPS Cat. #71266LIGHT-CHO Recombinant Cell Line (BPS Cat. # 79262)96-well tissue culture-treated white clear-bottom assay plateOne-Step luciferase assay system (BPS bioscience # 60690) or other luciferase reagents for measuring firefly luciferase activityLuminometer
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