性做久久久久久坡多野结衣-性做久久久久久久久浪潮-性欲影院-性影院-国产精品线路一线路二-国产精品兄妹在线观看麻豆

武漢原生原代生物醫(yī)藥科技有限公司

PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells

時(shí)間:2021-11-11 閱讀:261
分享:

PriCells: Primary Culture of Pulmonary Arterial Smooth Muscle Cells                            

1.Primary cultures of Pulmonary Arterial Smooth Muscle Cells (PASMCs) were isolated from human main pulmonary artery.
2.A segment of the main pulmonary artery was excised and placed in a sterile 10-cm dish containing primary cell culture media.
3.The segment was stripped of adventitia with a sterile forceps.
4.The main pulmonary artery segment was then cut longitudinally to open the vessel, and the endothelial layer was removed by gentle rubbing with a cell scraper.
5.The vessel was then cut into 2-mm segments, inverted, and placed on a collagen-coated 35-mm tissue culture dish.
6.A drop of primary cell culture media containing 10% fetal bovine serum, primary cell culture supplements, antibiotics, and antimycotics was then added, and the cells were grown overnight at 37°C in a humidified atmosphere with 5% CO2-95% air.
7.The next day, an additional 2 ml of complete medium were added.
8.The growth medium was subsequently changed every 2 days.
9.When SMC islands could be observed under the microscope, the tissue segment was removed and the individual cell islands were subcloned.
10.Identity was confirmed as PASMCs by immunostaining (>99% positive) with SMC actin. This was taken as evidence that cultures were not contaminated with fibroblasts or endothelial cells.
11.All cultures for subsequent experiments were maintained in primary cell culture media containing 10% fetal bovine serum, primary cell culture supplements, antibiotics, and antimycotics at 37°C in a humidified atmosphere with 5% CO2-95% air.

References
1.Chamley-Campbell, J., Campbell, G., and Ross, R.: The Smooth Muscle in Cell Culture, Physiol. Res. 59, 1, 1979.
2.Richardson, M., Taylor, D., Casey, M., MacDonald, P., and Stull, J.: Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture, In Vitro Cell. Dev. Biol. 23, 21, 1987.
3.Eskin, S., Sybers, H., Lester, J., Navarro, L., Gotto, A., and DeBakey, M.: Human Smooth Muscle Cells Cultured From Atherosclerotic Plaques and Uninvolved Vessel Wall, In Vitro 17 (8), 713, 1981

會(huì)員登錄

×

請(qǐng)輸入賬號(hào)

請(qǐng)輸入密碼

=

請(qǐng)輸驗(yàn)證碼

收藏該商鋪

X
該信息已收藏!
標(biāo)簽:
保存成功

(空格分隔,最多3個(gè),單個(gè)標(biāo)簽最多10個(gè)字符)

常用:

提示

X
您的留言已提交成功!我們將在第一時(shí)間回復(fù)您~
撥打電話(huà) 產(chǎn)品分類(lèi)
在線(xiàn)留言
主站蜘蛛池模板: 博爱县| 响水县| 沾益县| 南召县| 江阴市| 普兰店市| 南郑县| 拉孜县| 济阳县| 山东省| 同江市| 明溪县| 蒲江县| 建宁县| 富宁县| 资源县| 北京市| 赫章县| 佛坪县| 巍山| 灵石县| 宜都市| 英德市| 长泰县| 乐陵市| 天台县| 泽库县| 井研县| 抚松县| 西吉县| 清徐县| 岳阳市| 绍兴市| 穆棱市| 涪陵区| 上思县| 安泽县| 荔波县| 称多县| 泗阳县| 综艺|