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小鼠胚胎成纖維細(xì)胞C3H/10T1/2, Clone 8

種屬	小鼠
別稱	C3H/10T1/2-clone8; C3H/10T1/2 CL8; C3H10T1/2 clone8; C3H10T1/2CL8; 10T1/2(clone8); 10T1/2; C3H10T1-2; C3H10T1/2; C3H-10T1/2; C3H 10T1/2; C3H/10T1/2
組織來源	胚胎
疾病	肉瘤
傳代比例/細(xì)胞消化	1:2傳代，消化2-3分鐘
培養(yǎng)基配置	MEM培養(yǎng)基；10%胎牛血清；1%雙抗
簡(jiǎn)介	該細(xì)胞系是由C. Reznikoff等（1972）從C3H系小鼠胚胎細(xì)胞分離。此細(xì)胞對(duì)過度長(zhǎng)滿的細(xì)胞有絲分裂抑制非常敏感，在同源小鼠中不產(chǎn)生腫瘤，無自然轉(zhuǎn)化的背景，也不含明顯內(nèi)源性轉(zhuǎn)化鼠類白血病或肉瘤病毒。
形態(tài)	成纖維細(xì)胞樣
生長(zhǎng)特征	貼壁生長(zhǎng)
倍增時(shí)間	每周 2 至 3 次
致瘤性	No, in immunosuppressed mice.Yes, in semisolid medium.
培養(yǎng)條件	氣相：空氣，95% ；二氧化碳，5%。溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。
凍存條件	凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號(hào)JY-H040
保藏機(jī)構(gòu)	ATCC; CCL-226
產(chǎn)品使用	于科學(xué)研究，不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用。

nonetheless, a possible role exists in defining the optimal treatment strategy for many patients. Historically, tumour burden has been assessed using imaging. In particular, CT scans have been used to evaluate both the number and size of metastases as well as the number of organs involved. These methods are now often complemented by metabolic tumour burden, measured