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產品名稱:SU-DHL-10 人彌漫大B細胞淋巴瘤細胞、SU-DHL-10 人彌漫大B細胞淋巴瘤細胞、SU-DHL-10 人彌漫大B細胞淋巴瘤細胞、SU-DHL-10 人彌漫大B細胞淋巴瘤細胞;
種屬 | 人 |
別稱 | Su-DHL-10; SUDHL10; Sudhl10; SUDHL-10; SuDHL 10; Stanford University-Diffuse Histiocytic Lymphoma-10; DHL10 |
組織來源 | 淋巴結 |
疾病 | 大細胞淋巴瘤 |
傳代比例/細胞消化 | 1:2傳代 |
培養基配置 | RPMI1640 培養基;10%胎牛血清;1%雙抗 |
簡介 | established from the pleural effusion of a 25-year-old man with B-cell non-Hodgkin lymphoma (B- NHL), described at the time as diffuse histiocytic lymphoma; cell line carries EZH2 Y641F mutation (own results); assigned to GCB-like lymphoma subtype (germinal center B-cell) |
形態 | 淋巴母細胞樣 |
生長特征 | 懸浮生長 |
倍增時間 | ~40h |
STR | D5S818: 11, 12 D13S317: 10, 11 D7S820: 8, 11 D16S539: 10, 11 vWA: 17 THO1: 8, 9.3 TPOX: 8 CSF1PO: 10, 13 Amelogenin: XYTPOX: 8 CSF1PO: 10, 13 |
培養條件 | 氣相 :空氣 ,95% ;二氧化碳 ,5%。 溫度 :37攝氏度 ,培養箱濕度為70%-80%。 |
凍存條件 | 凍存液 :90%FBS ,DMSO 10%, 或使用非程序凍存液 :貨號JY-H040 |
保藏機構 | ATCC; CRL-2963 |
備注 | 該細胞為懸浮細胞 ,請注意離心收集細胞懸液 ,請勿直接倒掉細胞培養液。 |
產品使用 | 于科學研究 ,不可作為動物或人類疾病的治療產品使用。 |
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