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人B淋巴細胞GM12878

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供貨周期 現(xiàn)貨 規(guī)格 T25培養(yǎng)瓶x1 1.5ml凍存管x2
貨號 BFN60810358 應用領域 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè)
主要用途 僅供科研
青旗(上海)生物技術發(fā)展有限公司,總部位于上海浦東新區(qū),依托本地高校資源,逐步發(fā)展成為以生物技術為主的研發(fā)、生產(chǎn)、培訓為一體的綜合化產(chǎn)業(yè)平臺,在標準化細胞庫建立及細胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營原代細胞、細胞系、ELISA試劑盒、感受態(tài)細胞和HPLC檢測等科研產(chǎn)品與服務。我們秉承對用戶負責的態(tài)度,以對科研的高度嚴謹,以嚴格的質量控制,為廣大生物醫(yī)學科研用戶提供更優(yōu)質的服務!

細胞名稱

B淋巴細GM12878

img1

貨物編碼

BFN60810358

產(chǎn)品規(guī)格

T25培養(yǎng)x1

1.5ml凍存x2

細胞數(shù)量

1x10^6

1x10^6

保存溫度

37

-198

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科   3

 

培養(yǎng)體系

90%DMEM(不含丙酮酸鈉+10%FBS+1%三抗

培養(yǎng)溫度

37

二氧化碳濃度

5%

簡介

B淋巴細GM12878取自女性外周血。

注釋

Part of: CEPH/Utah pedigree cell line collection.

Part of: ENCODE project common cell types; tier 1.

Part of: Genetic Testing Reference Material (GeT-RM) samples.

Part of: Genome in a Bottle (GIAB) consortium samples.

Part of: International Genome Sample Resource (1000 genomes project) cell lines.

Registration: CEPH Families Reference Panel; 146302.

Registration: National Institute of Standards and Technology, Standard Reference Materials; HG001.

Population: Utah residents with ancestry from Northern and Western Europe.

Transformant: NCBI_TaxID; 10376; Epstein-Barr virus (EBV).

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: DNA methylation analysis.

Omics: Deep RNAseq analysis.

Omics: Genome sequenced.

Omics: H3K27ac ChIP-seq epigenome analysis.

Omics: H3K27me3 ChIP-seq epigenome analysis.

Omics: H3K36me3 ChIP-seq epigenome analysis.

Omics: H3K4me1 ChIP-seq epigenome analysis.

Omics: H3K4me2 ChIP-seq epigenome analysis.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: H3K79me2 ChIP-seq epigenome analysis.

Omics: H3K9ac ChIP-seq epigenome analysis.

Omics: H3K9me3 ChIP-seq epigenome analysis.

Omics: H4K20me1 ChIP-seq epigenome analysis.

Omics: Mitochondrial genome sequenced.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Derived from sampling site: Peripheral blood.

基因突變

CYP2C19 p.Pro227Pro (c.681G>A); cryptic splice acceptor activation; allele CYP2C19*2 (Coriell).

HLA信息

/

STR信息

/

參考文獻

PubMed=21418647; DOI=10.1186/gb-2011-12-3-r25

Morcos L., Ge B., Koka V., Lam K.C., Pokholok D.K., Gunderson K.L., Montpetit A., Verlaan D.J., Pastinen T.

Genome-wide assessment of imprinted expression in human cells.

Genome Biol. 12:R25.1-R25.14(2011)

 

PubMed=23325432; DOI=10.1101/gr.147942.112

Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.

Dynamic DNA methylation across diverse human cell lines and tissues.

Genome Res. 23:555-567(2013)

 

PubMed=23676674; DOI=10.1038/nature12223

Wu L., Candille S.I., Choi Y., Xie D., Jiang L., Li-Pook-Than J., Tang H., Snyder M.

Variation and genetic control of protein abundance in humans.

Nature 499:79-82(2013)

 

PubMed=24185094; DOI=10.1038/nbt.2728

Selvaraj S., Dixon J.R., Bansal V., Ren B.

Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing.

Nat. Biotechnol. 31:1111-1118(2013)

 

PubMed=26621101; DOI=10.1016/j.jmoldx.2015.08.005

Pratt V.M., Everts R.E., Aggarwal P., Beyer B.N., Broeckel U., Epstein-Baak R., Hujsak P., Kornreich R., Liao J., Lorier R., Scott S.A., Smith C.H., Toji L.H., Turner A., Kalman L.V.

Characterization of 137 genomic DNA reference materials for 28 pharmacogenetic genes: a GeT-RM collaborative project.

J. Mol. Diagn. 18:109-123(2016)

 

PubMed=30485824; DOI=10.1016/j.celrep.2018.10.096

Papp E., Hallberg D., Konecny G.E., Bruhm D.C., Adleff V., Noe M., Kagiampakis I., Palsgrove D., Conklin D., Kinose Y., White J.R., Press M.F., Drapkin R., Easwaran H., Baylin S.B., Slamon D., Velculescu V.E., Scharpf R.B.

Integrated genomic, epigenomic, and expression analyses of ovarian cancer cell lines.

Cell Rep. 25:2617-2633(2018)

 

PubMed=31273215; DOI=10.1038/s41597-019-0116-4

Osorio D., Yu X., Yu P., Serpedin E., Cai J.J.

Single-cell RNA sequencing of a European and an African lymphoblastoid cell line.

Sci. Data 6:112-112(2019)

 

PubMed=31401124; DOI=10.1016/j.jmoldx.2019.06.007

Gaedigk A., Turner A., Everts R.E., Scott S.A., Aggarwal P., Broeckel U., McMillin G.A., Melis R., Boone E.C., Pratt V.M., Kalman L.V.

Characterization of reference materials for genetic testing of CYP2D6 alleles: a GeT-RM collaborative project.

J. Mol. Diagn. 21:1034-1052(2019)

 青旗(上海)生物技術發(fā)展有限公司,總部位于上海浦東新區(qū),依托本地高校資源,逐步發(fā)展成為以生物技術為主的研發(fā)、生產(chǎn)、培訓為一體的綜合化產(chǎn)業(yè)平臺,在標準化細胞庫建立及細胞藥物前端模型方面成果顯著。公司生產(chǎn)經(jīng)營原代細胞、細胞系、ELISA試劑盒、感受態(tài)細胞和HPLC檢測等科研產(chǎn)品與服務。我們秉承對用戶負責的態(tài)度,以對科研的高度嚴謹,以嚴格的質量控制,為廣大生物醫(yī)學科研用戶提供更優(yōu)質的服務!

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