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Source

Rabbit

Applications

WB,IHC,IF,ELISA

Reactivity

Human

Dilution

WB: 1:500 - 1:2000. IHC: 1:100 - 1:300. IF: 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

Phospho-BLM (T99) Polyclonal Antibody detects endogenous levels of BLM protein only when phosphorylated at T99.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

The antiserum was produced against synthesized peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99. AA range:65-114

Uniprot No

P54132

Alternative names

BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ2; RecQ protein-like 3

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Background

Bloom syndrome RecQ like helicase(BLM) Homo sapiens The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008],

Other

BLM, Bloom syndrome protein; RecQ2; RecQ protein-like 3

Mol.Wt (Da)

159000

Concentration

1 mg/ml