Source

Rabbit

Applications

IF,WB,IHC,ELISA

Reactivity

Human,Mouse,Rat

Dilution

IF: 1:50-200 WB: 1:500 - 1:2000. IHC: 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

Storage

-20°C/1 year

Specificity

Akt Polyclonal Antibody detects endogenous levels of Akt protein.

Source/Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen

The antiserum was produced against synthesized peptide derived from human AKT1/2/3. AA range:281-330

Uniprot No

P31749/P31751/Q9Y243

Alternative names

AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B

Form

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Background

AKT serine/threonine kinase 1(AKT1) Homo sapiens The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Other

AKT1/AKT2/AKT3, RAC-alpha serine/threonine-protein kinase/RAC-beta serine/threonine-protein kinase/RAC-gamma serine/threonine-protein kinase

Mol.Wt (Da)

55716

Concentration

1 mg/ml