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Plant Soil 植物Pb、Cu重金屬脅迫 文章發表
NMT是基因功能的活體檢測技術，已被31位諾貝爾獎得主所在單位，及北大、清華、中科院使用。?
NMT歷*的今天
2016年11月13日，中科院煙臺海岸帶研究所駱永明、李連禎利用NMT在Plant and Soil 上發表了標題為Determining the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique的研究成果。
期刊：Plant and Soil
主題：使用掃描離子選擇電極技術確定濕地植物根表面離子（Pb2+，Cu2+和Cd2+）的通量
標題：Determining the fluxes of ions (Pb2+, Cu2+ and Cd2+) at the root surface of wetland plants using the scanning ion-selective electrode technique
影響因子：3.410
檢測指標：Pb2+、Cu2+、Cd2+流速
作者：中科院煙臺海岸帶研究所駱永明、李連禎
英文摘要
Measuring specific ion fluxes from different regions of the root under practical physiological conditions is crucial for understanding metal uptake mechanisms by plants.
We developed and tested a neutral carrier-based liquid-membrane Pb2+ and Cu2+ ion selective microelectrode (ISME) to investigate ion-transport processes along the roots of three common wetland plant species.
The Pb2+ and Cu2+ ISME exhibited a Nernstian response with Pb2+ and Cu2+ activities as low as 1.0 nM and 1.0 μM in deionized water and simulated soil solution, respectively. Phragmites australis had a region of Cu2+ release for approximately the first 200 μm, while it exhibited Pb2+ and Cd2+ outward net flux up to the first 500 μm. Although in older sections of the root of Phragmites australis there were areas of influx of Cu2+, Pb2+ and Cd2+, the overall influx was much smaller than that of Typha latifolia or Canna indica. Such a reduced uptake and/or an increased efflux of metal ions across the root-cell plasma-membrane might explain the higher resistance of Phragmites australis to metals, at least in part.
The Pb2+ and Cu2+ ISMEs are shown to permit detailed investigation of heavy-metal ion transport in plant roots, especially for plants used for phytoremediation.
中文摘要（谷歌機翻）
在實際的生理條件下，測量來自根不同區域的比離子通量對于理解植物對金屬的吸收機理至關重要。
我們開發并測試了基于中性載體的液膜Pb2+和Cu2+離子選擇性微電極（ISME），以研究沿三種常見濕地植物物種根部的離子遷移過程。
在去離子水和模擬土壤溶液中，Pb2+和Cu2+的ISME表現出Nernstian響應，Pb2+和Cu2+的活性分別低至1.0 nM和1.0μM。蘆葦在大約初的200μm處具有Cu2+釋放區域，而在初的500μm處表現出Pb2+和Cd2+向外凈通量。盡管在蘆葦根部的較老部分中有Cu2+，Pb2+和Cd2+的流入區域，但總流入量比香蒲或印度香豆小得多。穿過根細胞質膜的這種減少的金屬離子吸收和/或增加的金屬離子外流可能至少部分解釋了蘆葦對金屬的更高抗性。
研究表明，Pb2+和Cu2+ ISME可以詳細研究植物根特別是用于植物修復的植物中重金屬離子的轉運。
Fig. 3 Measurement of fluxes (outward positive) of Cd2+,Pb2+ and Cu2+ (mean ± standard error) across the root tips of three common wetland plant species, Typha latifolia (a) Canna indic (b) and Phragmites australis (c), using Cd2+,Pb2+ and Cu2+ ion selective microelectrodes and the scanning ion-selective electrode higher metal influx in the root regions, especially in the meristematic and elongation zone (Fig. 3).