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Contents and Storage

• 3-7 x 106 A549-ASC-NLRP1 cells in a cryovial or shipping flask IMPORTANT: If cells provided in a cryovial are not frozen upon arrival, contact InvivoGen immediately.

• 1 ml of Blasticidin(10 mg/ml). Store at 4°C or at -20°C.*

• 1 ml of Normocin™ (50 mg/ml), a formulation of three antibiotics active against mycoplasmas, bacteria and fungi. Store at -20°C.*

*The expiry date is specified on the product label. Handling Frozen Cells Upon Arrival Cells must be thawed immediately upon receipt and grown according to handling procedures (as described on the next page) to ensure the best cell viability and proper assay performance. Note: Avoid freezing cells upon receipt as it may result in irreversible damage to the cell line.

Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedures. IMPORTANT: For cells that arrive in a shipping flask please refer to the enclosed ‘cell recovery procedure’.

Cell Line Stability

Cells will undergo genotypic changes resulting in reduced responsiveness over time in normal cell culture conditions. Genetic instability is a biological phenomenon that occurs in all stably genetically engineered cells. Therefore, it is critical to prepare an adequate number of frozen stocks at early passages. To ensure maximum efficiency, do not passage A549-ASC-NLRP1 cells more than 20 times.

Quality Control

• The expression and functionality of ASC::GFP and NLRP1 has been verified by Western blot, flow cytometry and fluorescence microscopy, respectively.

• The stability of this cell line for 20 passages following thawing has been verified.

• A549-ASC-NLRP1 cells are guaranteed mycoplasma-free

Applications:

Screening of NLRP1 inflammasome activating stimuli
Real-time monitoring of ASC-dependent inflammasome assembly
Rapid and visual screening of drugs targeting the NLRP1 inflammasome by fluorescence microscopy

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