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綠茶代謝物分析
Green Tea Metabolite Profiling by LC-MS
Conditions
Column: ACE Excel 1.7 C18-Amide
Dimensions: 100 x 2.1 mm
Part Number: EXL-1712-1002U
Mobile Phase:
A: 0.01% formic acid in H2O
B: 0.01% formic acid in MeCN
Time(mins) | %B |
0.0 | 3 |
2.5 | 10 |
8.0 | 100 |
8.5 | 3 |
10.0 | 3 |
Flow Rate: 0.5 mL/min
Detection: Exactive accurate mass MS system ESI in negative ion mode
Analytes between m/z 70-800 monitored
Sample: Metabolites from green tea extracted into cold water by vortexing for 20mins. Samples filtered prior to injection onto column and modular Accela LC system
