BD Purified Rat Anti-Mouse CD16/CD32 553141  

純化大鼠抗小鼠CD16/CD32小鼠Fc阻滯劑

Brand:BD Pharmingen™

Alternative Name:FcγRIII/FcγRII; Fcgr3/Fcgr2

Reactivity:Mouse (QC Testing)

Isotype:Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ

Immunogen:Mouse BALB/c Macrophage J774

Application:Blocking, Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation (Reported)

Concentration:0.5 mg/ml

Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status:RUO

準備和存儲

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

推薦的實驗流程

To specifically stain cells bearing FcγII and FcγIII receptors for flow cytometric analysis: Incubate cell suspension with this antibody (≤ 1 μg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent.

To reduce Fc receptor-mediated binding by antibodies of interest or Fc receptor-mediated binding by PE-CY5 tandem dye conjugates to FcγII and FcγIII receptor-bearing mouse cells for flow cytometric analysis:

1. Preincubate cell suspension with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (eg, ≤ 1 μg/million cells in 100 μl) at 4?C for 5 minutes.

2. Add antibody of interest directly to preincubated cells in the presence of Mouse BD Fc Block™ (ie, Mouse BD Fc Block™ need not be washed off before staining cells).

3. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Mouse BD Fc Block™ (eg, rat IgG2b, κ).

BD Purified Rat Anti-Mouse CD16/CD32 553141