BD 人iPSC分選和分析試劑盒562626

BD Stemflow™ Human Induced Pluripotent Stem Cell Analysis and Sorting Kit

BD Stemflow™ 人誘導多能干細胞分選與分析試劑盒

產品概述

本試劑盒包含 小鼠單克隆抗體偶聯物組合，用于 人誘導多能干細胞（hiPSC）培養物 及 重編程過程中的細胞 的分選與分析。

主要應用

• hiPSC 分選：從異質性細胞群中分選 hiPSC

• 重編程過程監測：鑒定處于重編程不同階段的細胞

• 表面標志物分析：基于 3 種關鍵表面標志物 進行流式檢測

試劑盒組成

1. 抗體偶聯物（靶向 hiPSC 特異性表面標志物）

2. 同型對照（Isotype Controls）

3. BD™ CompBead Plus（補償微球，用于流式補償調節）

實驗流程

1. 細胞準備：按標準重編程方案獲得 hiPSC 或重編程中細胞

2. 抗體標記：使用試劑盒內抗體偶聯物進行染色

3. 流式分選/分析：通過 FACS 或流式細胞術 鑒定目標細胞群

優勢特點

? 高特異性：優化的抗體組合確保 hiPSC 精準分選
? 標準化操作：包含對照及補償微球，減少實驗誤差
? 兼容性強：適用于 hiPSC 培養、重編程效率評估 等研究

BD 人iPSC分選和分析試劑盒562626

The BD Stemflow™ Human iPSC Sorting and Analysis Kit contains a combination of mouse monoclonal antibody conjugates for the sorting and analysis of induced human pluripotent stem cell (hiPSC) cultures and cells in the process of being reprogrammed.  Following established reprogramming protocols, hiPSCs are sorted or analyzed from a heterogeneous cell culture utilizing the included antibody conjugates to three cell surface markers.  The antibody specificities that are provided in this kit and the cell populations they can identify are listed in the table below.  The kit also includes Isotype Controls and BD™ CompBead Plus.