TREM2 Effector Reporter Cell
| I. Background | |
TREM2是免疫球蛋白超家族的單次跨膜受體，主要表達于巨噬細胞、未成熟單核細胞 衍生的樹突狀細胞、破骨細胞和小膠質細胞上。TREM2 包含免疫球蛋白 V 型結構的胞外 域、跨膜域和沒有信號傳導基序的短胞內結構域。它通過 DNAX 激活蛋白 10 和 12 （DAP10 和 DAP12）發出信號。單個 TREM2 分子可以同時與 DAP10 和 DAP12 相互作 用。 | |
| II. Description | |
TREM2 Effector Reporter 報告基因藥靶模型很好的模擬了體內 TREM2 的信號轉導過程， 原理見下圖所示。
Figure 1. TREM2 Effector Reporter 細胞模型原理圖 | |
| III. Introduction | |
| Expressed gene: | TREM2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1 μg/ml Puromycin+10 μg/ml Blasticidin+800 μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
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Figure 3. Dose Response of TREM2 Agonist Ab in TREM2 Effector Reporter Cells (C14). | |
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