CCR3/CHO
| I. Background | |
CCR3 基因（C-C 趨化因子受體 3 基因）是編碼一類 G 蛋白偶聯(lián)受體的關(guān)鍵基因，其 編碼的 CCR3 蛋白在免疫調(diào)節(jié)與炎癥反應(yīng)中發(fā)揮核心作用。該受體主要表達(dá)于嗜酸性粒細(xì) 胞、嗜堿性粒細(xì)胞及 Th2 淋巴細(xì)胞表面，能夠特異性結(jié)合 Eotaxin（CCL11）、Eotaxin-3 （CCL26）等 CC 類趨化因子，形成信號(hào)傳導(dǎo)通路。當(dāng)趨化因子與 CCR3 結(jié)合時(shí)，會(huì)激活 G 蛋白偶聯(lián)的級(jí)聯(lián)反應(yīng)，引發(fā)胞內(nèi)鈣離子流動(dòng)，促使細(xì)胞沿化學(xué)濃度梯度定向遷移至炎癥 部位。 | |
| II. Introduction | |
| Host Cell: | CHO |
| Expressed gene: | CCR3 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10% FBS+5 μg/ml Puromycin |
| Storage: | Liquid nitrogen |
| Application(s): | Binding assay for CCR3 |
| Transducer: | Gi |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Disease: | Hamster Chinese ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
Figure 1. Recombinant CCR3/CHO stably expressing CCR3.
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