| CBP74173 | |
| I. Background | |
| Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells. | |
| II.Introduction | |
| Expressed gene: | hIL18 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | IL18; IGIF; IL-18; IL-1g; IL1F4; interleukin 18 |
| Freeze Medium: | 90% FBS+10%DMSO |
| Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin+800ug/ml hygromycin+10ug/ml blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
Figure 1.Dose Response of Recombinant IL-18 in IL-18 Effector Reporter Cells(C12). | |
Figure 2.Inhibition of hIL18-Induced Reporter Activity By Recombinant Human IL18 BP Protein in hIL18 Effector Reporter Cells(C12). | |
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