| I. Background | |
| 腫瘤壞死因子-α（TNF-α）是一種分子量為 26 kDa 的跨膜蛋白，其前肽在 TNF-α-轉(zhuǎn)換 酶（TACE）裂解時(shí)以可溶性 17 kDa 分子的形式分泌。雖然活化的巨噬細(xì)胞是 TNF-α的主 要來(lái)源，但它也可以由多種其他細(xì)胞產(chǎn)生，如成纖維細(xì)胞、星形膠質(zhì)細(xì)胞、平滑肌細(xì)胞、 角化細(xì)胞和多種腫瘤細(xì)胞，包括 B 細(xì)胞淋巴瘤、乳腺癌和結(jié)腸癌。 TNF-α作為腫瘤促進(jìn)因子，參與腫瘤發(fā)生的所有步驟，包括腫瘤的轉(zhuǎn)化、增殖、血管 生成、侵襲和轉(zhuǎn)移。TNF α信號(hào)傳導(dǎo)也是免疫系統(tǒng)的重要組成部分，可抑制腫瘤發(fā)生，阻止病毒復(fù)制，是一種內(nèi)源性熱原，可誘導(dǎo)發(fā)燒和細(xì)胞凋亡。 | |
| II. Introduction | |
| Expressed gene: | Membrane Anchored TNFα |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5μg/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
Figure 1. Membrane Anchored TNFα Cell (Adherent) flow cytometry data.
Figure 2. Dose response of TNFα Abs in ADCC Bioassay Effector Cell (High Affinity)-Fcγ-NFAT-Jurkat (C36) With Membrane Anchored TNFα Cell (Adherent, C12).
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