mouse ADCC Bioassay Effector Cell Line mouse ADCC Bioassay Effector Cell Line I. Background | |
抗體依賴的細胞介導的細胞毒性作用（ADCC，antibody-dependent cell-mediated cytotoxicity ）是指抗體的 Fab 段結合病毒感染的細胞或腫瘤細胞的抗原表位，其 Fc 段與殺 傷細胞（NK 細胞、巨噬細胞等）表面的 FcR 結合，介導殺傷細胞直接殺傷靶細胞，它是 抗腫瘤的治療性抗體藥物發生作用的一種作用重要機制。 | |
| II. Description | |
Mouse ADCC Bioassay Effector Cell 報告基因藥靶模型很好的模擬了體內 ADCC 的信號轉導過程，原理見下圖所示。
Figure 1. Mouse ADCC Bioassay Effector Cell 模型原理圖 | |
| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: | FcγRⅣ |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+800 μg/ml hygromycin+1 μg/ml puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
Figure 2. Dose response of Anti-hCD20-mlgG2a in Mouse ADCC Bioassay Effector Cell (C3).
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