Cell Line Name：

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

KIT W557_K558del results in the deletion of 2 amino acids in the juxtamembrane domain (exon 11) of the Kit protein from amino acids 557 to 558 (PMID: 12918066). W557_K558del results in constitutive activation of Kit and increased Erk phosphorylation, and leads to increased invasiveness and expression of ETV1 in cell culture (PMID: 25239608, PMID: 26936919).

1.WB of c-Kit W557_K558del-V654A/BaF3

2.Sanger of c-Kit W557_K558del-V654A/BaF3

Figure 2. c-Kit W557_K558del-V654A/BaF3 V654A

Figure 3. c-Kit W557_K558del-V654A/BaF3 W557_K558del

3. Anti-proliferation assay

Figure 4. CTG Proliferation Assay of BaF3 C-KIT W557_ K558del/V654A(C8).