CBP73279 | |
| I. Introduction | |
Cell Line Name： | c-Kit N822K/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
Mycoplasma Status: | Negative |
| II.Background | |
KIT N822K lies within the activation loop in the protein kinase domain of the Kit protein (PMID: 24205792). N822K results in constitutive activation of Kit, increased Erk1/2 and Stat3 phosphorylation, is transforming in culture (PMID: 24205792, PMID: 31484543), leads to mislocalization of Kit to endolysosomes (PMID: 31484543), and has been identified as a secondary mutation associated with imatinib-resistance (PMID: 18488160). | |
| III. Representative Data | |
1.WB of c-Kit N822K/BaF3
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2. Sanger of c-Kit N822K/BaF3
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3. Anti-proliferation assay
Figure 3. CTG Proliferation Assay of BaF3 c-KIT N822K(C4). | |
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