CBP73206 | |
| I. Introduction | |
Cell Line Name： | c-Kit D816V/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
Human cKit with D816V substitution, GenBank Accession No. NM_000222, a.a. 544-end with N-terminal His- tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa. | |
| III. Representative Data | |
1.WB of c-Kit D816V/BaF3
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2. Sanger of c-Kit D816V/BaF3
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3. Anti-proliferation assay | |
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Figure 3 Anti-proliferation assay of three reference compounds on the c-Kit D816V/BaF3. | |
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