| CBP73278 | |
| I. Introduction | |
Cell Line Name： | BCR-ABL1 [Q252H/T315I] /BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
ABL1, ABL proto-oncogene 1, non-receptor tyrosine kinase, plays a role in regulation of cell growth through ERK5, Rac/JNK, and Stat1/3 pathways (PMID: 10373409). Fusion proteins of BCR-ABL1 resulting in constitutive kinase activity have been reported in various cancers (PMID: 18851712, PMID: 30125955) and a number of Abl1 mutations in the context of BCR-ABL1 have been demonstrated to confer drug resistance (PMID: 24456693). | |
| III. Representative Data | |
1.WB of BCR-ABL1 [Q252H/T315I] /BaF3
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2. Sanger of BCR-ABL1 [Q252H/T315I] /BaF3
Figure 2. BCR-ABL1 [Q252H/T315I] /BaF3 Fusion
Figure 3. BCR-ABL1 [Q252H/T315I] /BaF3 Q252H Mutation
Figure 4. BCR-ABL1 [Q252H/T315I] /BaF3 T315I Mutation | |
3. Anti-proliferation assay
Figure 5 .CTG Proliferation Assay of BaF3 BCR-ABL1 T315I Q252H Cells (C1). | |
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