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NUCLEOSIL® 4000-7 PEI

Silica-based HPLC phase for anion exchange chromatography of proteins and peptides

Features

Base material NUCLEOSIL® silica, particle size 7 µm, pore size 4000 Å
polymeric, covalently bonded polyethyleneimine network, weakly basic anion exchanger

Ion exchange capacity 0.15 mmol/g; protein binding capacity 61 mg BSA/g;
pH stability 2–8.5; max. working pressure 250 bar

Separation principle: reversible adsorption of negatively charged substances to positively charged groups on the exchanger material and their subsequent displacement by either increasing ionic strength or pH changes in the phase

Eluent in column methanol

High selectivity for numerous proteins: e.g., β-lactoglobulins A and B, two proteins differing in just two amino acids, can be separated in only 10 minutes; biological activity of purified proteins is preserved

Good binding and desorption kinetics for nucleotides as well

For more applications click here.

Ordering information

Length ->

125 mm

CC guard columns*

NUCLEOSIL® 4000-7 PEI

EC analytical columns

4 mm ID

720402.40

721091.40

* CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359). Guard column cartridges in packs of 2, other columns in packs of 1