NUCLEOGEL® RP

Polymer-based reversed phase HPLC phases
for biochemical applications

Features

Polystyrene resin cross-linked with divinylbenzene, available particle sizes 5 μm and 8 μm,
available pore sizes 100 Å and 300 Å ? USP L21
pH working range 1–13, max. working pressure 180 bar
eluent in column acetonitrile – water

Small pore columns for reversed phase separation of small molecules such as pharmaceuticals with basic properties, e.g., organic heterocycles; also suited for separation of nucleosides and nucleotides up to 5000 daltons; allow gradient as well as isocratic elution

Wide pore columns especially recommended for large biomolecules (proteins, peptides etc.),
higher background hydrophobicity compared to silica phases

For more applications click here.

萬古霉素vancomycin的定量分析

色譜柱：VA 150×4.6mm NUCLEOGEL RP 100-8
流動相：A:8%(v/v) acetonitrile-0.02M borate buffer PH 8.0
       B:16%(v/v) acetonitrile-0.02M borate buffer PH 8.0  梯度洗脫：0-* B in 17.5min
流  速：0.5毫升/分鐘
檢測器：UV 235nm

蛋白的分析

色譜柱：VA 50mm×4.6mm  NUCLEOGEL RP 300-8
流動相：A:0.1%TFA 水溶液   梯度洗脫：20-60%   B,22min
流  速：1.5毫升/分鐘
檢測器：UV 220nm
樣  品：1.Ribonuclease  2.Insulin  3.cytochrome c  4.Iysozyme  5.bovine serum albumin6.Myoglobin  7.Ovalbumin

NUCLEOGEL RP C18分析擬交感神經胺

色譜柱：VA 150/4.6 NUCLEOGEL 80-10 C18
流動相：A:0.1M 氫氧化胺：乙腈=68:32  B:0.1M氫氧化胺：乙腈=54:46  梯度洗脫0-* B,5min
流  速：1.0毫升/分鐘    進樣量：20 ul
溫  度：室溫             檢測器：UV 230nm
樣  品：1.Phenylpropanolamine   2.Pseudoephedrine   3.Amphetamine   4.Methamphetamine

Ordering information

Length ->

50 mm

150 mm

250 mm

Guard columns*

Valco type analytical columns      

NUCLEOGEL® RP 100-5 (particle size 5 µm, pore size 100 Å)

4.6 mm ID

719454

719455

719542

NUCLEOGEL® RP 100-8 (particle size 8 µm, pore size 100 Å)

4.6 mm ID

719456

719520

719542

NUCLEOGEL® RP 300-5 (particle size 5 µm, pore size 300 Å)

4.6 mm ID

719459

719542

NUCLEOGEL® RP 300-8 (particle size 8 µm, pore size 300 Å)

4.6 mm ID

719460

719542

* Valco type guard column cartridges measure 5 x 3 mm, require guard column holder B (REF 719539) and are supplied in packs of 2. Columns are supplied in packs of 1.