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【文獻標題】Replacing fish oil with palm oil: Effects on mRNA expression of fatty acid transport genes and signalling factors related to lipid metabolism in Nile tilapia (Oreochromis niloticus)

【作者】Christian Larbi Ayisi,Jin-Liang Zhao,Xue-Ming Hua,et.al

【作者單位】上海海洋大學（Shanghai Ocean University）

【文獻中引用產品】

魚過氧化物酶體增殖物激活受體α(PPAR-α)ELISA試劑盒

魚過氧化物酶體增殖物激活受體β(PPARβ)ELISA試劑盒

魚脂蛋白酯酶（LPL）ELISA試劑盒

魚脂肪酸結合蛋白3（FABP3）ELISA試劑盒

魚金屬忍耐蛋白(MTP)ELISA試劑盒

魚可溶性肝X受體(LXR)ELISA試劑盒

魚脂肪酸延長酶5(ELOVL5)ELISA試劑盒

魚葡萄糖6磷酸脫氫酶(G6PD)ELISA試劑盒

魚蘋果酸酶（ME）ELISA試劑盒

【關鍵詞】ELOVL 5, fatty acid metabolism, fatty acid transport, glucose 6-phosphate dehydrogenase, 

lipoprotein lipase, palm oil

【DOI】doi.org/10.1111/anu.12821

【出版期刊】《Aquaculture Nutrition》

【產品原文引用】

Enzyme activities

Enzyme-linked immunosorbent assay (ELISA) was used to measure the enzyme activities of PPAR-α, PPAR-β, LPL, FABP-3, MTP, LXR, ELOVL 5, 6GPD, G6PD and ME with a commercial kit from Shanghai Ding Biological Technology Company Limited (Shanghai, China). A ground glass homogenizer was used to homogenize liver (0.5-1.0 g) on ice. The homogenates were centrifuged (110 000 g, 20 min at 4°C), and the clear phase between the top layer and the pellets was used for the analysis. At 450 nm, an ELISA microplate reader was used to measure the optical density (OD) of samples in a 96-well plate. According to the manufacturer’s instructions, a standard curve was generated and the standard diversity calculated using Excel 2003. Enzyme activity units (IU), defined as moles of substrate, were converted into product per minute at assay temperature.