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針式組合PH微電極
The microelectrode is ready to use. Carefully unwind the tape and remove the probe from the
protective glass tube. The white translucent sleeve, which covers the hole, should be moved
down

針式組合PH微電極

Calibration
The electrode can be held by hand when measuring pH. It has been standardized in two (2) pHbuffers. Follow the procedure recommended by the manufacturer of your pH meter forcalibration.

針式組合PH微電極

Optimum Response Time: Optimum response time will be obtained after the probe has been
exercised in two (2) buffer solutions. Fill one beaker with a pH 4.01 buffer or equivalent and a
second beaker with a pH 6.86 buffer or equivalent. Hold the microelectrode and touch the pH 4
.01 buffer surface allowing 5 seconds for equilibration, and then touch the pH 6.86 buffer surface
in the same0 manner. Do this several times. It is not necessary to rinse the probe with distilled
water because the carry-over is negligible

針式組合PH微電極

Handling
The outer reference chamber of the microelectrode can be refilled when necessary with the 3 M
KCl solution, using the bulb pipette. Hold the electrode at a 60 degree angle (approx.) and
slowly insert the tip into the fill hole of the electrode.
Be careful not to apply pressure against the inner glass capillary tube.
Slowly fill the reference chamber. If filling is done too quickly, air may be trapped in the
chamber causing reference solution to squirt out of the fill hole instead of entering and filling the
reference chamber.
Cleaning
When using the microelectrode in solutions containing protein, it should be soaked in an enzyme
cleaning solution such as Terg-a-zyme (Alconox, Inc.) or a chromic/sulfuric acid glass cleaning
solution for a couple of minutes after each use to remove the protein from the glass and the
reference junction. This will prolong the useful life of the microelectrode