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【文獻標題】Emerging contaminant 1,3,6,8-tetrabromocarbazole induces oxidative damage and apoptosis during the embryonic development of zebrafish (Danio rerio)

【作者】Jingwen Zhang，Cheng Zhang，Zhongkun Du，et.al

【作者單位】山東農(nóng)業(yè)大學（Shandong Agricultural University）

【文獻中引用產(chǎn)品】

斑馬魚8羥基脫氧鳥苷（8-OHdG）ELISA試劑盒

斑馬魚抑肽酶（AP）ELISA試劑盒

斑馬魚總蛋白（TP）ELISA試劑盒

斑馬魚脂質(zhì)過氧化物酶（LPO）ELISA試劑盒

抗氧化酶（AOE）ELISA試劑盒

【關鍵詞】Polyhalogenated carbazoles，Zebrafish，Developmental toxicity，Antioxidant enzymes，Apoptosis

【DOI】doi.org/10.1016/j.scitotenv.2020.140753

【影響因子(IF)】5.92

【出版期刊】《Science of the Total Environment》

【產(chǎn)品原文引用】

Chemicals

1368-TBCZ (C12H5Br4N, CAS 55119-09-0, purity N95%) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Dimethyl sulfoxide (DMSO, 99.9% pure, Sigma, Missouri, USA) was used to prepare stock solutions. Kaitong Chemical Reagent Co., Ltd. (Tianjin,China) and Solarbio Science & Technology Co. (Beijing, China) provided the other chemicals (analytical grade). We used kits from Nanjing Jiancheng Bioengineering Research Institute (Jiangsu), Hengyuan Biotechnology Co., Ltd. (Shanghai), Solar Technology Co., Ltd. (Beijing,China) to measure total protein and lipid peroxidation levels, three measures of antioxidant activity changes, 8-OHdG content level, and apoptosis rate. The primers, reagents, and kits for gene expression measurement were purchased from CoWin Biosciences Co., Ltd. (Beijing,China) and Sangon Biotechnology Co., Ltd. (Shanghai, China)

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