BALB/3T3 clone A31小鼠胚胎成纖維細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和*培養條件!BALB/3T3 clone A31小鼠胚胎成纖維細胞
BALB/3T3 clone A31小鼠胚胎成纖維細胞 的詳細介紹
BALB/3T3 clone A31小鼠胚胎成纖維細胞
| ATCC? Number: | CCL-163? | Price: | $269.00 |
| Designations: | BALB/3T3 clone A31 | | Depositors: | S Aaronson | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Mus musculus （mouse） | | Morphology: | fibroblast
 | | Source: | Organ: embryo
Strain: BALB/c
Cell Type: fibroblast | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| | Isolation: | Isolation date: 1968 | | Applications: | testing [92346] [92389]
transfection host （Roche FuGENE? Transfection Reagents） | | Virus Susceptibility: | Herpes simplex virus
Vesicular stomatitis virus
Human poliovirus 1 | | Tumorigenic: | No | | Reverse Transcript: | negative | | Cytogenetic Analysis: | modal number = 78; range = 62 to 109.
The stemline number is hypotetraploid. Most of the cells contained only ocentric or acrocentric chromosomes. | | Age: | embryo; 14 to 17 day gestation | | Comments: | The BALB/3T3 clone A31 is one of several cell lines （see ATCC CCL-164, BALB/3T12） developed by S.A. Aaronson and G.T. Todaro in 1968 from disaggregated 14- to 17-day-old BALB/c mouse embryos [PubMed: 4301006].The cells are extremely sensitive to contact inhibition of cell division, grow at a high dilution, exhibit a low saturation density and are highly susceptible to transformation in tissue culture by the oncogenic DNA virus SV40 and murine sarcoma virus [PubMed: 4306523].
Tested and found negative for ectromelia virus （mousepox）. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C
Growth Conditions: The serum used is calf serum, NOT fetal calf serum. The depositor recommended calf serum because fetal calf serum causes transformation and loss of contact inhibition. |
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