CRL-6323 B16F1 小鼠黑色素瘤細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和*培養條件
CRL-6323 B16F1 小鼠黑色素瘤細胞 的詳細介紹
CRL-6323 B16F1 小鼠黑色素瘤細胞
| ATCC? Number: | CRL-6323? | Price: | $275.00 |
| Designations: | B16-F1 | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Mus musculus （mouse） | | Morphology: |
| | Source: | Organ: skin
Strain: C57BL/6J
Disease: melanoma | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| | Applications: | transfection host （Roche FuGENE? Transfection Reagents） | | Tumorigenic: | Yes | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C | | Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
Medium Renewal: Every 2 to 3 days | | Preservation: | Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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