CRL-1572 NRK-52E 大鼠腎細胞， ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件
CRL-1572 NRK-52E 大鼠腎細胞， ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和*培養條件
CRL-1572 NRK-52E 大鼠腎細胞 的詳細介紹
CRL-1572 NRK-52E 大鼠腎細胞
| ATCC? Number: | CRL-1571? | Price: | $331.00 |
| Designations: | NRK-52E | | Depositors: | JE DeLarco | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Rattus norvegicus （rat） | | Morphology: | epithelial
| | Source: | Organ: kidney
Disease: normal | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| | Applications: | transfection host （Roche FuGENE? Transfection Reagents） | | Receptors: | epidermal growth factor （EGF）; multiplication stimulating activity （MSA） | | Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide （CO2）, 5% | | Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.02% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh culture medium, aspirate and dispense into new culture vessels.
Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week | | Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature | | Related Products: | Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2030 | | References: | 22713: de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor （EGF） receptors and the effect of mouse sarcoma virus transformation. J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450
23197: Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742 |
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