HTB-20 BT-474 人乳腺導管癌細胞

 的詳細介紹

 HTB-20 BT-474 人乳腺導管癌細胞

ATCC® Number:  HTB-20™        
Designations:  BT-474  
Depositors:   EY Lasfargues  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent, patchy (The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent) 
Organism: Homo sapiens (human)  
Morphology: epithelial

 HTB-20 BT-474 人乳腺導管癌細胞Source: Organ: mammary gland; breast 
Tissue: duct 
Disease: ductal carcinoma 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
HTB-20 BT-474 人乳腺導管癌細胞
Virus Susceptibility: mouse mammary tumor virus (RIII-MuMTV) 
Tumorigenic: Yes  
DNA Profile (STR): Amelogenin: X 
CSF1PO: 10,11 
D13S317: 11 
D16S539: 9,11 
D5S818: 11,13 
D7S820: 9,12 
THO1: 7 
TPOX: 8 
vWA: 15,16 
Cytogenetic Analysis: The cell line is aneuploid human female (XO usually), with most chromosome counts in the hypertetraploid range. Several chromosomes (N11, N13, and N22) are absent, and others are clearly under-represented (N9, N14, and N15) with respect to the other normal chromosomes. Chromosome N7 tends towards over-representation in several karyotypes. Some of the missing normal chromosomes are represented by their involvement in the nine stable marker chromosomes: der(14)t(14;?)(q32,?), unknown, iso(13q), der(6)t(6;7)(q21;q21), der(11)t(11;?)(14;?), del(11)(p11), unknown, unknown, der(2)t(2;?)(p21;?). Several of the latter were reported by E. Lasfargues, et al. [22542] 
Isoenzymes:  AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 1 
Me-2, 0 HTB-20 BT-474 人乳腺導管癌細胞
PGM1, 1 
PGM3, 1 
Age:  60 years *  
Gender:  female  
Ethnicity:  Caucasian  
Comments: The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Subculturing:  Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. 
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended 
Medium Renewal: 2 to 3 times per week HTB-20 BT-474 人乳腺導管癌細胞
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
purified DNA:ATCC HTB-20D 
References: 22542: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572
22660: Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035
32275: Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 
 
 HTB-20 BT-474 人乳腺導管癌細胞

MLE-12 小鼠肺上皮細胞
saos-2 人成骨肉瘤細胞
MA104 非洲綠猴胚胎腎上皮細胞
ball-1 細胞
nci-h226 人肺鱗癌細胞
MCA38 小鼠結腸癌細胞
LTPA 小鼠胰腺癌細胞 
NCI-H23 人非小細胞肺癌細胞
293.00     人胚腎細胞

 HTB-20 BT-474 人乳腺導管癌細胞
293FT    人胚腎細胞-用于慢病毒包裝
293TN    人胚腎細胞--用于慢病毒包裝
293AV    人胚腎細胞-用于腺病毒包裝
293T    人胚腎細胞
208F    大鼠成纖維細胞
786-O    人腎透明細胞腺癌細胞
A172    人腦膠質母細胞瘤細胞
A549    人肺癌細胞 細胞系購買
AGS    人胃癌細胞
BEL7402 人肝癌細胞系
AZ-521    人胃癌細胞
B16F0    小鼠黑色素瘤細胞
Bel-7402    人肝癌細胞
Bel-7404    人肝癌細胞
BGC-823    人胃癌細胞
C6    大鼠腦膠質瘤細胞
SW480    人結腸腺癌細胞
CaCo-2    人結腸癌細胞
Cho-K1    倉鼠卵巢細胞亞株
COS-7    非洲綠猴SV40轉化的腎細胞
GES-1    人胃上皮細胞
HCT-8    人回盲腸癌細胞
HeLa    人宮頸癌細胞
Hepa1-6    小鼠肝癌細胞
HepG2    人肝癌細胞
hFOB1.19    人永生化成骨細胞
HGC-27    人胃癌細胞
Hs 746T    人胃癌細胞
HT-29    人結腸癌細胞
KB    人口腔表皮樣癌細胞
LoVo    人結腸癌細胞
LLC-PK1    豬腎近曲小管上皮細胞
MCF-10A    人正常乳腺上皮細胞
MCF-7    人乳腺癌細胞
MDA-MB-231    人乳腺癌細胞
MGC80-3    人胃癌細胞
MKN-45    人胃癌細胞
NCI-H460    人大細胞肺癌細胞
NIH-3T3    小鼠成纖維細胞
NUGC-3    人胃癌細胞
Raw264-7    小鼠單核細胞白血病細胞
SGC-7901    人胃癌細胞
SMMC-7721    人肝癌細胞
SNU-638    人胃癌細胞
SNU-739    人肝癌細胞
Tca8113    人舌鱗癌細胞    細胞系購買
U251    人腦膠質瘤細胞
U-373MG    人腦神經膠質瘤細胞
U-87 MG    人腦星形膠質母細胞瘤
BT-474    人乳腺導管癌細胞
PANC-1    人胰腺癌細胞
HEK293    人胚腎細胞
Hep3B    人肝癌細胞
HL-7702    人正常肝細胞
Raw-Blue    小鼠單核細胞白血病細胞
WEHI-231    小鼠B淋巴細胞
OPM-2    人骨髓瘤細胞
NCI-H929    人骨髓瘤細胞

HTB-20 BT-474 人乳腺導管癌細胞
U266    人骨髓瘤細胞
EJ    人膀胱癌細胞
HOC    大鼠肝卵圓細胞
RWPE-1    人正常前列腺上皮細胞
CAL 27    人舌鱗癌細胞
Calu-3    人肺腺癌細胞
Eca-109    人食管癌細胞
DLD-1    人結直腸腺癌上皮細胞
5637.00     人膀胱癌細胞
SK-OV-3    人卵巢癌細胞
GBC-SD    人膽囊癌細胞
BEL7402 人肝癌細胞系
SW620    人結腸癌細胞
MRC-5    人胚肺成纖維細胞     細胞系購買
PC9    人肺癌細胞
PC14    人肺癌細胞
BxPC    人原位胰腺腺癌細胞
PK（15）    豬腎上皮細胞
MDCK    狗腎上皮細胞
3D4/21    豬肺泡巨噬細胞
 HTB-20 BT-474 人乳腺導管癌細胞 
MHCC-97L    人肝癌細胞（低轉移）
MHCC-97H    人肝癌細胞系（高轉移）
MiaCaPa-2    人胰腺癌細胞
HN13    口腔鱗狀細胞癌細胞
Bend3    小鼠腦微血管內皮細胞
QBC939    人膽管癌細胞
Ishikawa    人子宮內膜癌細胞
A498    人腎癌細胞
143B    
WEHI-231    小鼠B淋巴細胞
BN    
P9R    小鼠骨髓基質細胞
P9    小鼠骨髓基質細胞
F98    大鼠腦膠質瘤細胞
NCIH1299    人非小細胞肺癌細胞
NCIH1650-M3    人非小細胞肺癌細胞
293LVT    人胚腎細胞--用于慢病毒包裝
EC9706    人食管癌細胞
CNE    人鼻咽癌細胞
H322    人非小細胞肺癌細胞
Panc O2    
S8    BEL7402 人肝癌細胞系
R5    細胞系購買
Pig MSC    豬骨髓間質干細胞
HCAEC    人冠狀動脈內皮細胞
marc-145    猴胚胎腎上皮細胞
3D4/21    豬肺泡巨噬細胞
PK（15）    豬腎上皮細胞
MADB-106    大鼠乳腺癌細胞
Sf9    昆蟲卵巢細胞
DADY    
HUVEC[HUV-EC-C]    人臍靜脈內皮細胞
PC14    人肺癌細胞
PC9    人肺癌細胞
Eca-109    人食管癌細胞
CAL 27    人舌鱗癌細胞
HOC    肝卵圓細胞
EJ    人膀胱癌細胞

HTB-20 BT-474 人乳腺導管癌細胞
U266    人骨髓瘤細胞
293-mTLR5    人胚腎細胞
Raw-Blue    小鼠單核細胞白血病細胞
HL-7702    人正常肝細胞
Hep3B    人肝癌細胞
U-373MG    人腦神經膠質瘤細胞
Tca-8113    人舌鱗癌細胞
SNU-739    人肝癌細胞
SNU-638    人胃癌細胞
NUGC-3    人胃癌細胞
NIH-3T3    小鼠成纖維細胞
MKN-45    人胃癌細胞
LLC-PK1    豬腎近曲小管上皮細胞
Hs 746T    人胃癌細胞
Hepa1-6    小鼠肝癌細胞
HCT-8    人回盲腸癌細胞
BEL7402 人肝癌細胞系
HaCaT    人永生化表皮細胞
GES-1    人胃上皮細胞
B16-F0    小鼠黑色素瘤細胞
AZ-521    人胃癌細胞
786-O    人腎透明細胞腺癌細胞
208F    大鼠成纖維細胞
293TN    人胚腎細胞--用于慢病毒包裝
293FT    人胚腎細胞-用于慢病毒包裝
GP-293    人胚腎細胞
293-L.P.    人胚腎細胞

 HTB-20 BT-474 人乳腺導管癌細胞