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重組小鼠白介素-6(rmIL-6)

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CYT-350重組小鼠白介素-6(rmIL-6) 2μg/10μg/1mg Prospec

 

 

Background:
 Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells.Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.

 
Description :
 Recombinant Murine IL-6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton.

Mouse IL-6 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 The protein was lyophilized from a concentrated (1mg/ml) solution containg 50 mM NH4HCO3, pH 8.0.

 
Solubility:
 It is recommended to reconstitute the lyophilized rmIL-6 in 100 mM acetic acid to 0.1-1.0 mg/mL to regain full activity, and can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized IL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-6 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less then 0.02 ng/ml, corresponding to a Specific Activity of 1 x 10 7IU/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of IL-6.

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 

 

 


佰易聚生物商城提供美國光譜醫學透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購!

 

 

 

 

 

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