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CYT-368

重組人轉化生長因子b3 （rHuTGFb3）

1ug/5ug

Prospec

Background:
	Transforming growth factor-betas （TGF betas） are multifunctional peptides that regulate growth and differentiation in a variety of cells. The transforming growth factor beta （TGF-beta） family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-beta isoforms （TGF-beta 1, -beta 2 and -beta 3） have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues Transforming growth factor （TGF） beta 3 is a new isoform of the TGF beta superfamily and is presumed to play an important role in wound repair and scarring. TGF-beta 3 is believed to be involved in osteoblast proliferation, chemotaxis, and collagen synthesis.
	Description :
	Recombinant Human TGF-b 3 produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25,445 Dalton. Recombinant Human TGF-b 3 is purified by standard chromatographic techniques.
	Physical Appearance:
	Sterile Filtered clear solution.
	Formulation:
	The protein solution （0.25mg/ml） contains 20% Ethanol and 0.12% Acetic acid.
	Stability:
	Recombinant Human TGF-beta 3 although stable at room temperature for 3 weeks, should be stored at 4⁰C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
	Purity:
	Greater than 99.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.
	Aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec’s TGF-beta 3 is fully biologically active when compared to standard. The ED50 as determined by the cell toxicity assay using the WHO Standard 98/608 as a direct comparison is < 0.05ng/ml, corresponding to a Specific Activity of 12.5 x 10⁶IU/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of Recombinant Human TGF-beta 3.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a calibrated solution of TGF-beta 3 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.