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北京西美杰科技有限公司
主營產品： 生物學檢測試劑盒,化學試劑,蛋白質氧化損傷,抗原抗體 |
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A5260,0050DNA Blood Isolation Spin-Kit（血液DNA提取試劑盒）
| 參考價 | 面議 |
具體成交價以合同協議為準
- 型號 A5260,0050
- 品牌
- 廠商性質 代理商
- 所在地 上海市
更新時間：2017-09-16 20:55:07瀏覽次數：754
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| HS-No. | 38220000 | |||
|---|---|---|---|---|
| Storage | RT | |||
| except for Proteinase K - Solution: +4°C | ||||
| • Kit for the DNA isolation from blood cells with spin minicolumns | ||||
| • Application of the DNA in PCR, cloning and sequencing | ||||
| • Maximum sample volume 100 μl | ||||
| • Binding capacity of the column 10 μg DNA | ||||
| • sufficient for 50 and 250 assays, respectively | ||||
| • Isolation of fragments in the range of 20 kb | ||||
The DNA Blood Isolation Spin-Kit is based on the ability of DNA to adsorb to silica surfaces in the presence of high concentrations of chaotropic salts. Blood cells are Iysed with the supplied lysis buffer, which contains chaotropic salts and a non-ionic detergent （TL buffer）. Cell lysis is additionally supported by the high-activity protease Proteinase K. In the next step, the whole sample is loaded onto a minicolumn with a special silica resin. The DNA binds to the silica resin, while contaminants （fragmented proteins, salts, etc.） pass freely through the column. Residual contaminations are removed by a washing step. Pure DNA is eluted with a low salt buffer or water and can be used in the following experiments without additional purification procedures （e.g. precipitation）.
