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27104-- QIAprep Spin Miniprep Kit (50T)

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QIAprep Spin Miniprep Kit

純化分子生物學級別純度質粒DNA,zui高可達 20 μg

  • 數分鐘內即可獲得即用型質粒DNA
  • 非樹脂技術,無需酚抽提
  • 分子生物學級別純度的質粒DNA,產量重現性高
 

  • Product Details
  •  

Principle

QIAprep spin columns contain a unique silica gel membrane that binds up to 20 µg DNA in the presence of a high concentration of chaotropic salt, and allows elution in a small volume of low-salt buffer. QIAprep membrane technology eliminates time consuming phenol-chloroform extraction and alcohol precipitation, as well as the problems and inconvenience associated with loose resins and slurries. High-purity plasmid DNA eluted from QIAprep spin columns is immediay ready to use - there is no need to precipitate, concentrate, or desalt.

Procedure

Plasmid purification using QIAprep Kits follows a simple bind-wash-elute procedure (see figure "The QIAprep procedure"). First, bacterial cultures are lysed and the lysates are cleared by centrifugation. The cleared lysates are then applied to the QIAprep module where plasmid DNA adsorbs to the silicagel membrane. Impurities are washed away and pure DNA is eluted in a small volume of elution buffer or water.

The QIAprep Spin MiniPrep Kit enables purification of up to 20 ug molecular biology grade plasmid DNA or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning. Versatile QIAprep spin columns can be used either in microcentrifuges or on vacuum manifolds (see figure "QIAprep Spin Column handling options"). The vacuum procedure provides simplified handling and faster sample processing. QIAprep spin columns can be vacuum processed using the QIAvac 24 plus, QIAvac 6S withQIAvac Luer Adapters, or any other commercial manifold with luer connectors. The QIAprep Spin Miniprep Kit can now also be fully automated on the QIAcube.

 Characteristics  
Format Spin columns
Purification module QIAprep spin columns
Throughput 1–24 samples
Preparation time

24 minipreps in 30 minutes

Equipment required Microcentrifuge or vacuum manifold; fully automatable using the QIAcube
Lysate clearing Centrifugation

 

Specifications

 
Capacity of column reservoir 800 µl
Minimum elution buffer volume 50 µl
Culture volume for high-copy plasmids 1–5 ml
Culture volume for low-copy plasmids/cosmids 1–10 ml

Applications

QIAprep Miniprep Kits provide reproducible yields of high-purity DNA suitable for use in most applications, including:

  • Automated fluorescent sequencing, including capillary sequencing
  • Radioactive sequencing
  • Restriction digestion (see figure "Complete digestion with various restriction enzymes")
  • Screening
  • Ligation and transformation
  • Transfection of robust cells

In addition to plasmid purification from Escherichia coli, QIAprep Kits can be used to purify plasmid DNA from Saccharomyces cerevisiae, Bacillus subtilis, and Agrobacterium tumefaciens. Contact QIAGEN Technical Services or your local distributor for protocols for these applications.

Images

QIAprep Spin Column handling options.Complete digestion with various restriction enzymes.QIAprep spin column.The QIAprep procedure.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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