抗連環(huán)蛋白β1(CTNNb1)多克隆抗體

HZB021Hu01 Anti-Catenin Beta 1 (CTNNb1) Polyclonal Antibody Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016) [ PROPERTIES ] Source: Polyclonal antibody preparation Host: Rabbit Purification: Antigen-specific Affinity Chromatography Traits: Liquid Concentration: 200µg/mL UOM: 100µg Applications: Loading Control of WB [ IMMUNOGEN ] Immunogen: RPB021Hu01-Recombinant Catenin Beta 1 (CTNNb1)抗連環(huán)蛋白β1(CTNNb1)多克隆抗體 [ ORGANISM SPECIES MORE ] React with: Mouse, rat, bovine; Other species have not been detected. [ APPLICATIONS ] Western blotting: 0.5-2ug/ml Optimal working dilutions must be determined by end user. [ FORMULATION ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant CTNNb1 disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). 抗連環(huán)蛋白β1(CTNNb1)多克隆抗體Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 4 oC for frequent use. Aliquot and store at -20 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is,抗連環(huán)蛋白β1(CTNNb1)多克隆抗體 incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. [ IDENTIFICATION ] A B Figure 1. Western Blot A. Sample: Recombinant CTNNb1, Human B. Lane1: Mouse Liver Tissue Lane2: Mouse Lung Tissue Primary Ab: 3µg/mL Rabbit Anti-Human CTNNb1 Ab Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SAA544Rb59)抗連環(huán)蛋白β1(CTNNb1)多克隆抗體HZ719Hu01    蛋白激酶Bβ(PKBβ)重組蛋白        Recombinant Protein Kinase B Beta (PKBb)    Homo sapiens (Human)
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