產品名稱：Glutathione Peroxidase Activity Assay Kit
產品價格：4788
產    地：BioVision
產品貨號：K762-100
產品規格：100 assays
其它產品：
Glutathione Reductase Activity Assay Kit 4788  BioVision K761-200 200 assays
產品說明：
Storage Conditions: -20°C
Shipping Conditions: gel pack
Kit Summary:
• Detection method- Absorbance （340 nm）
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum,erythrocyte lysates, as well as many other biological fluids etc.
• Species reactivity- Mammalian
• Application- The assay has adetection sensitivity ~ 0.5 mU/ml of GPx in samples.
Features & Benefits:
• Simple procedure; takes ~ 30-40 minutes
• Fast and convenient
Kit components:
• GPx Assay Buffer
• NADPH （lyophilized）
• Glutathione Reductase
• Glutathione （GSH, lyophilized）
• Cumene Hydroperoxide
• GPx Positive Control （lyophilized）
Description: Glutathione Peroxidase （GPx, EC 1.11.1.9） family of enzymes play important roles in the protection of organisms from oxidative damage. GPx converts reduced glutathione （GSH） to oxidized glutathione （GSSG） while reducing lipid hydroperoxides to their corresponding alcohols or free hydrogen peroxide to water. Several isozymes have been found in different cellular locations and with different substrate specificity. Low levels of GPx have been correlated with free radical related disorders. In BioVision’s Glutathione Peroxidase Activity Assay, GPx reduces Cumene Hydroperoxide while oxidizing GSH to GSSG. The generated GSSG is reduced to GSH with consumption of NADPH by GR. The decrease of NADPH （easily measured at 340 nm） is proportional to GPx activity. The assay can be used to measure all of the glutathione dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates with a detection sensitivity of ~ 0.5 mU/ml of GPx in samples.